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Histone H4 Antikörper (acLys12)

Reaktivität: Human, Maus, Ratte WB, IHC, IF, ELISA, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6254899
  • Target Alle Histone H4 Antikörper anzeigen
    Histone H4
    Bindungsspezifität
    • 33
    • 30
    • 29
    • 28
    • 19
    • 19
    • 18
    • 16
    • 15
    • 14
    • 14
    • 13
    • 12
    • 12
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    acLys12
    Reaktivität
    • 329
    • 251
    • 246
    • 17
    • 14
    • 12
    • 11
    • 10
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Human, Maus, Ratte
    Wirt
    • 366
    • 23
    • 2
    Kaninchen
    Klonalität
    • 339
    • 50
    • 2
    Polyklonal
    Konjugat
    • 212
    • 21
    • 14
    • 14
    • 14
    • 14
    • 14
    • 14
    • 7
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    Dieser Histone H4 Antikörper ist unkonjugiert
    Applikation
    • 210
    • 102
    • 99
    • 93
    • 56
    • 52
    • 48
    • 47
    • 41
    • 39
    • 33
    • 22
    • 22
    • 15
    • 13
    • 8
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)
    Spezifität
    Acetyl-Histone H4 (Lys12) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine12.
    Kreuzreaktivität
    Human, Maus, Ratte (Rattus)
    Aufreinigung
    affinity purification
    Immunogen
    The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.
    Isotyp
    IgG
  • Applikationshinweise
    WB: 1:500~1:2000 IHC: 1:50~1:200 IF 1:200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #104615 (Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #104615
    Datum
    22.09.2024
    Antigen
    Histone H4
    Chargennummer
    lk4553
    Validierte Anwendung
    Immunohistochemistry
    Positivkontrolle

    CD1 mouse brain fixed in 4% paraformaldehyde.

    Negativkontrolle

    Control slices were processed for each experimental procedure, omitting the primary antibody. In this case, overnight incubation was only performed using a diluent solution.

    Bewertung
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN6254899
    Sekundärantikörper
    Alexa Fluor 488 goat anti-rabbit (Invitrogen by Thermo Fisher Scientific, A11034, lot # 2380031), Alexa Fluor 488 donkey anti-rabbit (Invitrogen by Thermo Fisher Scientific A32790 lot # XJ357262).
    Full Protocol
    • SAMPLE ANALYZED: Old (> 18 months) CD1 mouse brain.
    • Mice were perfused with paraformaldehyde 4% in 0.1M phosphate buffer (PB) pH 7.4 and samples were post-fixed in the same fixative for 2 additional hours at room temperature. After fixation, samples were washed, dehydrated, and embedded in paraffin wax.
    • Following several washes in PBS, the brain was cut with a microtome (6 μm-thick sections) in parasagittal sections mounted on glass slides.
    • After paraffin removal, sections were incubated for 1 hour at room temperature in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot # 00583) to block non-specific binding sites. Sections were then incubated overnight at room temperature with the primary antibody anti-Histone H4 (acLys12) (ABIN6254899) at 1:50, 1:100, and 1:200, in PBS-BSA (Sigma, A7906)-PLL (Sigma, P1524).
    • Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 minutes (95–100°C) in 10 mM sodium citrate buffer (pH 6.0). After 20 minutes of spontaneous cooling, sections were washed (distilled water, 2 times for 5 min each; followed by PBS for 5 min).
    • Sections were washed 3×5 min in 0.01M PBS.
    • Sections were then incubated with the anti-rabbit secondary antibody Alexa Fluor 488, 1:500, in 0.1M PBS, for 1 hour at room temperature.
    • Sections were washed 3×5 min in 0.01M PBS.
    • Specimens were mounted in Fluoroshield (Sigma, F6182, lot # MKCB0153V).
    • Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 20× or 40× magnification.
    Anmerkungen

    Passed. The antibody works for IHC-P at 1:100 and 1:200 concentrations with or without microwave antigen retrieval. Microwave treatment increases the intensity of staining.

  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20 °C.Stable for 12 months from date of receipt
    Haltbarkeit
    12 months
  • Target
    Histone H4
    Abstract
    Histone H4 Produkte
    Hintergrund

    Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene: HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

    Molekulargewicht
    11kDa
    Gen-ID
    121, 504, 554
    UniProt
    P62805
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