Abscisic Acid Insensitive 5 (ABI5) Antikörper

Produktnummer ABIN4966067

Der Kaninchen Polyklonal anti-Abscisic Acid Insensitive 5 (ABI5) Antikörper wird verwendet zum Nachweis von Abscisic Acid Insensitive 5 (ABI5) in Proben von Arabidopsis thaliana. Er wurde validiert für IL und WB.

Kurzübersicht für Abscisic Acid Insensitive 5 (ABI5) Antikörper (ABIN4966067)

Target: Abscisic Acid Insensitive 5 (ABI5)

Reaktivität: Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunolocalization (IL), Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-ABI5 | Abscisic acid insensitive 5 (peptide antibody)

Kreuzreaktivität (Details): Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Brassica napus,Populus trichocarpa

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence

Anwendungsinformationen

Applikationshinweise: 1: 140 (IL), 1 : 200 (WB)

Kommentare:

ABI5 protein is present in very low levels therefore specific material should be used for analysis as well as most sensitive detection reagents.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water to each tube

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: Abscisic Acid Insensitive 5 (ABI5)

Hintergrund:

Background: ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39.

Additional information: MG132 is recommened to be added to extraction buffer as ABI5 is degraded by proteasome as well as homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here.

Molekulargewicht: 47 kDa

Gen-ID: 818199

UniProt: Q9SJN0