TNNI3 Antikörper (pSer23, pSer24)

Produktnummer ABIN6254118

Der Kaninchen Polyklonal Anti-TNNI3 Antikörper wurde für WB und ELISA validiert. Er ist geeignet, TNNI3 in Proben von Maus zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für TNNI3 Antikörper (pSer23, pSer24) (ABIN6254118)

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNNI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TNNI3 Antikörper

Bindungsspezifität: pSer23, pSer24

Spezifität: Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser23/24. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: human, non-human primate

Produktmerkmale: Our Troponin I (cardiac) Ser23/24 rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Troponin I (cardiac) Ser23/24 and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Aufreinigung: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 of mouse troponin 1, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-TNNI3 Antikörper (ABIN6254118)

Lin, Schmidt, Fritz, Jeong, Cammarato, Foster, Biesiadecki, McKinsey, Woulfe: "Site-specific acetyl-mimetic modification of cardiac troponin I modulates myofilament relaxation and calcium sensitivity." in: Journal of molecular and cellular cardiology, Vol. 139, pp. 135-147, (2020) (PubMed).

Woulfe, Ferrara, Pioner, Mahaffey, Coppini, Scellini, Ferrantini, Piroddi, Tesi, Poggesi, Jeong: "A Novel Method of Isolating Myofibrils From Primary Cardiomyocyte Culture Suitable for Myofibril Mechanical Study." in: Frontiers in cardiovascular medicine, Vol. 6, pp. 12, (2019) (PubMed).

Details zu TNNI3

Target: TNNI3 (Cardiac Troponin I (TNNI3))

Andere Bezeichnung: TNNI3

Hintergrund:

Synonyms: cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody

Description Logic: Our Troponin I (cardiac) Ser23/24 rabbit polyclonal phosphospecific primary antibody is produced . It detects Troponin I (cardiac) Ser23/24 and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: TNNI3

Molekulargewicht: 25 kDa

Gen-ID: 21954

UniProt: P48787