Elastase 3B Antikörper (AA 82-238)

Produktnummer ABIN6252899

Dieses Anti-Elastase 3B-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Elastase 3B in WB, ELISA, IF, FACS und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für Elastase 3B Antikörper (AA 82-238) (ABIN6252899)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Elastase 3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CELA3B-1257

Produktdetails anti-Elastase 3B Antikörper

Bindungsspezifität: AA 82-238

Aufreinigung: Protein G

Immunogen: A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 2-4 μg/mL, order BSA free format for coating

Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

IHC-P: 1-2 μg/mL for 30 min at RT

Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Andere Bezeichnung: CELA3B

Hintergrund: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Gen-ID: 23436

UniProt: P08861