Elastase 3B Antikörper (AA 82-238)

Produktnummer ABIN6252899

Dieser Anti-Elastase 3B Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von Elastase 3B in WB, ELISA, IF, FACS und IHC (p). Geeignet für Human, Ratte und Maus.

Kurzübersicht für Elastase 3B Antikörper (AA 82-238) (ABIN6252899)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Elastase 3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CELA3B-1257

Produktdetails anti-Elastase 3B Antikörper

Bindungsspezifität: AA 82-238

Aufreinigung: Protein G

Immunogen: A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 2-4 μg/mL, order BSA free format for coating

Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml

IF: 1-2 μg/mL

IHC-P: 1-2 μg/mL for 30 min at RT

Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Andere Bezeichnung: CELA3B

Hintergrund: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Gen-ID: 23436

UniProt: P08861