pan Keratin Antikörper

Produktnummer ABIN6252866

Dieses Anti-pan Keratin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von pan Keratin in IF, FACS, WB und IHC (p). Geeignet für Human.

Kurzübersicht für pan Keratin Antikörper (ABIN6252866)

Target: pan Keratin (panKRT)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser pan Keratin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-pan Keratin Antikörper

Aufreinigung: Protein A affinity chromatography

Immunogen: Recombinant full-length human KRT76 and KRT77 proteins were used as the immunogen for this pan Cytokeratin antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/10e6 cells in 0.1ml

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.25-0.5 μg/mL for 30 min at RT

Prediluted format: incubate for 30 min at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Pan Cytokeratin

Hintergrund: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody detects acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins: 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8), 56.5  kDa (CK10), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), 40  kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.

Gen-ID: 3848, 3850, 3851

UniProt: P04264, P12035, P19013, P13647, P48668, P05787, P13645, P02533, P19012, P08779, P08727