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Secretory Component Glycoprotein Antikörper

Dieses Anti-Secretory Component Glycoprotein-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Secretory Component Glycoprotein in IHC (p), IF, FACS und WB. Geeignet für Human und Ratte.
Produktnummer ABIN6252815

Kurzübersicht für Secretory Component Glycoprotein Antikörper (ABIN6252815)

Target

Secretory Component Glycoprotein

Reaktivität

Human, Ratte

Wirt

  • 3
Maus

Klonalität

  • 3
Monoklonal

Konjugat

  • 3
Dieser Secretory Component Glycoprotein Antikörper ist unkonjugiert

Applikation

  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Klon

ECM1-792
  • Aufreinigung

    Protein G affinity chromatography

    Immunogen

    Recombinant human protein was used as the immunogen for the Secretory Component Glycoprotein antibody.

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Optimal dilution of the Secretory Component Glycoprotein antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.2 mg/mL

    Buffer

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target

    Secretory Component Glycoprotein

    Hintergrund

    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

    Gen-ID

    1893

    UniProt

    Q16610
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