TIMP3 Antikörper (AA 175-211)

Produktnummer ABIN6252786

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch TIMP3 in WB, IHC, IP, FACS und IF. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund und Pferd.

Kurzübersicht für TIMP3 Antikörper (AA 175-211) (ABIN6252786)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TIMP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-TIMP3 Antikörper

Bindungsspezifität: AA 175-211

Aufreinigung: Protein A affinity chromatography

Immunogen: Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: use antibody at 0.5-1 μg/million cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IP: 1-2 μg/500 μg protein lysate

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Andere Bezeichnung: TIMP3

Hintergrund: TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

Gen-ID: 7078

UniProt: P35625