Igk Antikörper

Produktnummer ABIN6252720

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Igk in FACS, IHC, WB und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Igk Antikörper (ABIN6252720)

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Igk Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Klon: SPM558

Produktdetails anti-Igk Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human Ig kappa chain was used as the immunogen for this anti-Kappa antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The optimal dilution of the anti-Kappa antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Igk

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Andere Bezeichnung: Kappa

Hintergrund: This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Gen-ID: 3514

UniProt: P01601, P01834