ECM1 Antikörper

Produktnummer ABIN6252531

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ECM1 in IHC und IF. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für ECM1 Antikörper (ABIN6252531)

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ECM1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: SC05

Produktdetails anti-ECM1 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Secretory component protein isolated from human colostrum was used as the immunogen.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu ECM1

Target: ECM1 (Extracellular Matrix Protein 1 (ECM1))

Andere Bezeichnung: Extracellular Matrix Protein 1

Hintergrund: This antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory component. It does not react with the cell lines lacking this protein. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Gen-ID: 1893

UniProt: Q16610