CD16 Antikörper

Produktnummer ABIN6252333

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD16 in FACS, IF, WB und Func. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD16 Antikörper (ABIN6252333)

Target: CD16

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD16 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Functional Studies (Func)

Klon: HuNK2

Produktdetails anti-CD16 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Human peripheral blood lymphocytes from a NK-leukemia patient were used as the immunogen for the CD16 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Functional Activity (Order SAF formulation)

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Optimal dilution of the CD16 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu CD16

Target: CD16

Andere Bezeichnung: CD16

Hintergrund: Recognizes a protein of 50-65  kDa, identified as CD16 (Workshop IV, Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This mAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.

Gen-ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process