CD16 Antikörper

Produktnummer ABIN6252332

Dieses Anti-CD16-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD16 in FACS, IF und Func. Geeignet für Human.

Kurzübersicht für CD16 Antikörper (ABIN6252332)

Target: CD16

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD16 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Klon: HO-80

Produktdetails anti-CD16 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: PBL s from a NK-leukemia patient were used as the immunogen for the CD16 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Functional Activity (Order SAF formulation)

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Optimal dilution of the CD16 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu CD16

Target: CD16

Andere Bezeichnung: CD16

Hintergrund: It recognizes CD16 (Fcgamma,RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80  kDa. Two similar genes represent CD16, CD16A (Fcgamma,RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fcgamma,RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Gen-ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process