MLANA Antikörper

Produktnummer ABIN6252326

Produktdetails anti-MLANA Antikörper

Target: MLANA (Melan A (MLANA))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLANA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human Melan-A protein was used as the immunogen for this antibody.

Klon: M2-9E3

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: ELISA: order BSA-free format for coating

Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IP: 0.5-1 μg/500 μg protein lysate

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu MLANA

Target: MLANA (Melan A (MLANA))

Andere Bezeichnung: Melan-A (MLANA Produkte)

Synonyme: A930034P04Rik antikoerper, Mart1 antikoerper, MART-1 antikoerper, MART1 antikoerper, melan-A antikoerper, Mlana antikoerper, MLANA antikoerper

Hintergrund: This antibody recognizes a protein doublet of 20-22  kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Gen-ID: 2315

UniProt: Q16655