KRT15 Antikörper (C-Term)

Produktnummer ABIN6252303

Dieses Maus Monoklonal-Antikörper erkennt spezifisch KRT15 in WB, FACS, IF, IHC (fro) und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte und Rind (Kuh).

Kurzübersicht für KRT15 Antikörper (C-Term) (ABIN6252303)

Target: KRT15 (Keratin 15 (KRT15))

Reaktivität: Human, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: LHK15

Produktdetails anti-KRT15 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Protein G affinity chromatography

Immunogen: A 17 amino acid sequence from the C- terminal of human CK15 was used as the immunogen for the Keratin 15 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

WB: 0.5-1 μg/mL

Immunohistochemistry (FFPE & Frozen): 0.5-1 μg/mL for 30 min at RT

Optimal dilution of the Keratin 15 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu KRT15

Target: KRT15 (Keratin 15 (KRT15))

Andere Bezeichnung: Cytokeratin 15

Hintergrund: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

Gen-ID: 3866

UniProt: P19012