VEGF Antikörper

Produktnummer ABIN6252287

Dieser Anti-VEGF Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von VEGF in WB, IF und IHC (p). Geeignet für Human, Maus, Ratte, Hund und Kaninchen.

Kurzübersicht für VEGF Antikörper (ABIN6252287)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VEGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: VEGF-1063

Produktdetails anti-VEGF Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for the VEGF antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Optimal dilution of the VEGF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Andere Bezeichnung: VEGF

Hintergrund: This mAb recognizes proteins of 19-22  kDa (reducing) and 38  kDa-44  kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Gen-ID: 7422

UniProt: P15692