Biotin Antikörper

Produktnummer ABIN6252183

Dieser Maus Monoklonal Antikörper detektiert spezifisch Biotin in WB, IHC, FACS, IF, CM und Func.

Kurzübersicht für Biotin Antikörper (ABIN6252183)

Target: Biotin

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Biotin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Confocal Microscopy (CM), Functional Studies (Func)

Klon: SPM375

Produktdetails anti-Biotin Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Confocal Microscopy

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

In situ Nucleic Acid Hybridization

Western: blot 1-3 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.