RBP4 Antikörper (AA 74-182)

Produktnummer ABIN6252115

Der Maus Monoklonal anti-RBP4 Antikörper wird verwendet zum Nachweis von RBP4 in Proben von Human, Maus, Ratte, Kaninchen, Affe und Ziege. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für RBP4 Antikörper (AA 74-182) (ABIN6252115)

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reaktivität: Human, Maus, Ratte, Kaninchen, Affe, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RBP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: G4E4

Produktdetails anti-RBP4 Antikörper

Bindungsspezifität: AA 74-182

Aufreinigung: Protein G affinity chromatography

Immunogen: Retinol binding protein purified from human plasma was used as the immunogen for this RBP antibody. Its epitope localizes between amino acids 74-182.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

IF: 1-2 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Andere Bezeichnung: RBP

Hintergrund: Retinol binding protein a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. RBP1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.

Gen-ID: 5947

UniProt: P09455

Pathways: Regulatorische RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response