dsDNA Antikörper

Produktnummer ABIN6252090

Dieser Maus Monoklonal Antikörper detektiert spezifisch dsDNA in FACS, IF, IHC (p) und ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für dsDNA Antikörper (ABIN6252090)

Target: dsDNA (Double-Stranded DNA (dsDNA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser dsDNA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Klon: SPM603

Produktdetails anti-dsDNA Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.

Isotyp: IgG3 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunocytochemistry (Acetone-fixed): 0.5-1 μg/mL for 30 min at RT

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu dsDNA

Target: dsDNA (Double-Stranded DNA (dsDNA))

Andere Bezeichnung: Double Stranded DNA

Substanzklasse: Nucleotide

Hintergrund: Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.