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Granulocyte Marker Antikörper

Reaktivität: Human, Makake FACS, IF, IHC, WB Wirt: Maus Monoclonal BM-2 unconjugated
Produktnummer ABIN6252005
  • Target
    Granulocyte Marker
    Reaktivität
    Human, Makake
    Wirt
    • 12
    Maus
    Klonalität
    • 12
    Monoklonal
    Konjugat
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Unkonjugiert
    Applikation
    • 11
    • 11
    • 10
    • 9
    • 9
    • 2
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte Marker antibody.
    Klon
    BM-2
    Isotyp
    IgG1
  • Applikationshinweise
    Flow Cytometry: 0.5-1 μg/million cells

    IF: 1-2 μg/mL

    IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Granulocyte Marker antibody to be titered up or down for optimal performance.

    1. No special pretreatment is required for staining of formalin/paraffin tissues.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    Granulocyte Marker
    Hintergrund
    This antibody recognizes an unidentified antigen in the cytoplasm of mature granulocytes and can be used as a granulocyte marker in normal tissues or inflammatory processes. The granulocyte marker antibody shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. Clone BM-2 mAb reacts with early precursor and mature forms of human myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas.
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