Ferritin Antikörper (AA 38-165)

Produktnummer ABIN6252003

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Ferritin in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Ferritin Antikörper (AA 38-165) (ABIN6252003)

Target: Ferritin (FE)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ferritin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: FTLC1-1

Produktdetails anti-Ferritin Antikörper

Bindungsspezifität: AA 38-165

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin antibody (light chain specific).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Ferritin

Target: Ferritin (FE)

Andere Bezeichnung: Ferritin

Hintergrund: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Gen-ID: 2512

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis