Actin, Muscle Specific Antikörper

Produktnummer ABIN6251982

Dieser Maus Monoklonal Antikörper detektiert spezifisch Actin, Muscle Specific in FACS, IF und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Kaninchen und Ratte.

Kurzübersicht für Actin, Muscle Specific Antikörper (ABIN6251982)

Target: Actin, Muscle Specific

Reaktivität: Human, Kaninchen, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin, Muscle Specific Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: SPM160

Produktdetails anti-Actin, Muscle Specific Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Human myocardium extract was used as the immunogen for this Muscle Specific Actin antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

The optimal dilution of the Muscle Specific Actin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Actin, Muscle Specific

Target: Actin, Muscle Specific

Andere Bezeichnung: Muscle Specific Actin

Hintergrund: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Gen-ID: 59

UniProt: P62736