KRT1 Antikörper

Produktnummer ABIN6251964

Dieses Maus Monoklonal-Antikörper erkennt spezifisch KRT1 in IF, WB, FACS und IHC. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für KRT1 Antikörper (ABIN6251964)

Target: KRT1 (Cytokeratin HMW (KRT1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Klon: 34BE12

Produktdetails anti-KRT1 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/10e6 cells

IF: 0.5-1.0 μg/mL

WB: 0.5-1.0 μg/mL

IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HMW Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu KRT1

Target: KRT1 (Cytokeratin HMW (KRT1))

Andere Bezeichnung: HMW Cytokeratin

Hintergrund: This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.

Gen-ID: 3848

UniProt: P04264