CD56 Antikörper (Extracellular Domain)

Produktnummer ABIN6251899

Der Maus Monoklonal anti-CD56 Antikörper wird verwendet zum Nachweis von CD56 in Proben von Human, Ratte und Zebrafisch (Danio rerio). Er wurde validiert für FACS, IHC und IF.

Kurzübersicht für CD56 Antikörper (Extracellular Domain) (ABIN6251899)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reaktivität: Human, Ratte, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD56 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: SPM128

Produktdetails anti-CD56 Antikörper

Bindungsspezifität: Extracellular Domain

Aufreinigung: Protein G affinity chromatography

Immunogen: A membrane preparation of small cell lung carcinoma was used as the immunogen for this CD56 antibody. This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the CD56 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Andere Bezeichnung: CD56

Hintergrund: Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135  kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Gen-ID: 4684

UniProt: P13591