HCG alpha Antikörper

Produktnummer ABIN6251828

Der Maus Monoklonal anti-HCG alpha Antikörper (Klon CGA91-2) (ABIN6251828) detektiert spezifisch HCG alpha in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für HCG alpha Antikörper (ABIN6251828)

Target: HCG alpha

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: CGA91-2

Produktdetails

Aufreinigung: Protein G purified monoclonal antibody

Immunogen: Recombinant protein was used as the immunogen for this HCG-alpha antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Variations in protocols, secondaries and substrates may require the HCG-alpha antibody to be titered for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Aliquot and Store at 2-8C. Avoid freez-thaw cycles.

Antigendetails

Target: HCG alpha

Andere Bezeichnung: HCG-alpha

Hintergrund: This antibody reacts with a protein of ~13  kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).

Gen-ID: 1081

UniProt: P01215