Nucleoli Marker Antikörper

Produktnummer ABIN6251821

Der Maus Monoklonal anti-Nucleoli Marker Antikörper wird verwendet zum Nachweis von Nucleoli Marker in Proben von Human. Er wurde validiert für IF, FACS, IHC und ICC.

Kurzübersicht für Nucleoli Marker Antikörper (ABIN6251821)

Target: Nucleoli Marker

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Klon: NCM102-1

Produktdetails

Aufreinigung: Protein G affinity chromatography

Immunogen: A preparation of myeloid leukemia cell nuclei was used as the immunogen.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 0.5-1.0 μg/mL

Immunocytochemistry (Acetone-fixed): 0.5-1.0 μg/mL for 30 min at RT

IHC (Formalin-paraffin): 0.5-1.0 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the Nucleoli marker antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Antigendetails

Target: Nucleoli Marker

Hintergrund: The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]