EZH2 Antikörper (AA 260-295)

Produktnummer ABIN6244309

Der Kaninchen Polyklonal anti-EZH2 Antikörper (Klon RB51326) (ABIN6244309) detektiert spezifisch EZH2 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für EZH2 Antikörper (AA 260-295) (ABIN6244309)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EZH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB51326

Produktdetails anti-EZH2 Antikörper

Bindungsspezifität: AA 260-295

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse Ezh2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 260-295 amino acids from the Central region of Mouse Ezh2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Andere Bezeichnung: Ezh2

Hintergrund: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer, involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

Molekulargewicht: 85292

UniProt: Q61188

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation