SMURF2 Antikörper

Produktnummer ABIN6243825

Der Maus-Monoklonal Anti-SMURF2-Antikörper wurde für WB validiert. Er ist geeignet zum Nachweis von SMURF2 in Proben aus Human. }

Kurzübersicht für SMURF2 Antikörper (ABIN6243825)

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SMURF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 1826CT865-51-28

Produktdetails anti-SMURF2 Antikörper

Aufreinigung: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This SMURF2 antibody is generated from a mouse immunized with a recombinant protein of human SMURF2.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: WB: 1:2000-1:4000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu SMURF2

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Andere Bezeichnung: SMURF2

Hintergrund: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Molekulargewicht: 86196

UniProt: Q9HAU4