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HSP18.5 Antikörper

Dieses Anti-HSP18.5-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HSP18.5 in WB. Geeignet für Arabidopsis thaliana.
Produktnummer ABIN619791

Kurzübersicht für HSP18.5 Antikörper (ABIN619791)

Target

HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))

Reaktivität

Arabidopsis thaliana

Wirt

Kaninchen

Klonalität

Polyklonal

Applikation

Western Blotting (WB)
  • Kreuzreaktivität (Details)

    Not reactive in: no confirmed exceptions from predicted reactivity are currently known

    Homologie

    dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 18.5 kDa

    Aufreinigung

    serum

    Immunogen

    recombinant Arabidopsis thaliana Hsp18.5
  • Applikationshinweise

    1 : 1000 with standard ECL (WB)

    Kommentare

    hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 200 μL of sterile water.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target

    HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))

    Andere Bezeichnung

    HSP18.5

    Hintergrund

    HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein

    Molekulargewicht

    18.5 kDa
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