Histone 3 Antikörper

Produktnummer ABIN619545

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-Histone 3 Antikörper (ABIN619545) detektiert spezifisch Histone 3 in WB, IF, ICC und ChIP-qPCR. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Arabidopsis thaliana, Oryza sativa, Tomato, Mais (Zea mays), Chlamydomonas reinhardtii, Phaeodactylum tricornutum, Phalaenopsis equestris, Nicotiana benthamiana, Botrytis cinerea (Grey Mold), Cabbage, Camellia sinensis, Chickpea, Gerste, Chlamydomonas acidophila, Cucumis sativus, Cucurbita pepo, Physcomytrium Patens, Potato, Red pepper, Salicornia europaea, Solanum sogarandinum, Stevia rebaudiana und Vicia faba.

Kurzübersicht für Histone 3 Antikörper (ABIN619545)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Arabidopsis thaliana, Oryza sativa, Tomato, Mais (Zea mays), Chlamydomonas reinhardtii, Phaeodactylum tricornutum, Phalaenopsis equestris, Nicotiana benthamiana, Botrytis cinerea (Grey Mold), Cabbage, Camellia sinensis, Chickpea, Gerste, Chlamydomonas acidophila, Cucumis sativus, Cucurbita pepo, Physcomytrium Patens, Potato, Red pepper, Salicornia europaea, Solanum sogarandinum, Stevia rebaudiana, Vicia faba

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ChIP quantitative PCR (ChIP-qPCR)

Produktdetails anti-Histone 3 Antikörper

Verwendungszweck: Anti-H3 | Histone H3 (rabbit antibody) (nuclear marker)

Kreuzreaktivität (Details): Arabidopsis thaliana, Botrytis cinerea, Brassica oleracea, Camellia sinensis, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Oryza sativa, Phalaenopsis equestris, Phaeodactylum tricornutum, Physcomitrium patens, Salicornia europaea, Stevia rebaudiana, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Brachypodium distachyon,Brassica napus,Emiliania huxleyi,Fagopyrum tataricum Gaertn.,Glycine max,Nannochloropsis gaditana,Nicotiana tabacum,Malus domestica,Medicago sativa,Oryza sativa,Ostreococcus sp.,Pinus pinaster,Pisum sativum,Porphyridium purpureum,Saccharum sp.,Sorghum bicolor,Triticum aestivum,Vitis vinifera,Volvox sp.

Aufreinigung: Serum

Immunogen: KLH-conjugated synthetic peptide derived from known H3 sequences

Anwendungsinformationen

Applikationshinweise: 1 : 100-1 : 500 (ICC), 2 μL of antibody/500 μL solution (ChIp-qPCR), 1: 500 (IF), 1 : 5000 (WB)

Kommentare:

Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-Histone 3 Antikörper (ABIN619545)

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

van der Linde, Hemetsberger, Kastner, Kaschani, van der Hoorn, Kumlehn, Doehlemann: "A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases." in: The Plant cell, Vol. 24, Issue 3, pp. 1285-300, (2012) (PubMed).

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: H3

Hintergrund:

Background: Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Additional information: Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

Molekulargewicht: 15 | 17 kDa

Gen-ID: 830164

UniProt: P59169, P59226, Q9FXI7