Biotin Antikörper (HRP)
Kurzübersicht für Biotin Antikörper (HRP) (ABIN619361)
Target
Wirt
Klonalität
Konjugat
Applikation
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Aufreinigung
- affinity purified
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Immunogen
- Biotin
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Isotyp
- IgG
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Applikationshinweise
- This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500 - 1:5 000 for Immunohistochemistry 1:200 - 1:5000 for ELISA/Western blot
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Handhabung
- Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- Biotin
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Substanzklasse
- Chemical
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Hintergrund
- Goat anti-biotin is a secondary antibody conjugated to HRP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
Target
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