FTL Antikörper (AA 38-165) (CF®594)

Produktnummer ABIN6191688

Der Maus Monoklonal Anti-FTL-Antikörper wurde für IHC, IF und FACS validiert. Er ist geeignet, FTL in Proben von Human zu detektieren.

Kurzübersicht für FTL Antikörper (AA 38-165) (CF®594) (ABIN6191688)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FTL Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: FTL-1389

Produktdetails anti-FTL Antikörper (CF®594)

Bindungsspezifität: AA 38-165

Verwendungszweck: Mouse Monoclonal anti-Ferritin, Light chain (FTL/1389), CF594 Conjugate

Produktmerkmale: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FTL protein fragment (aa 38-165) (exact sequence is proprietary)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.1-0.2 μg/mL

• Flow cytometry 0.1-0.2 μg/million cells Immunofluorescence 0.1-0.2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Optimal dilution for a specific application should be determined by user

Kommentare:

HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Andere Bezeichnung: Ferritin, Light chain

Hintergrund: Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3

Molekulargewicht: 19-25 kDa

Gen-ID: 2512, 433670

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis