Elastase 3B Antikörper (AA 82-238) (CF®488A)

Produktnummer ABIN6191572

Dieses Anti-Elastase 3B-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Elastase 3B in WB, ELISA, Coat, IF und FACS. Geeignet für Human.

Kurzübersicht für Elastase 3B Antikörper (AA 82-238) (CF®488A) (ABIN6191572)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Elastase 3B Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), ELISA, Coating (Coat), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: CELA3B-1811

Produktdetails anti-Elastase 3B Antikörper (CF®488A)

Bindungsspezifität: AA 82-238

Verwendungszweck: Mouse Monoclonal anti-CELA3B / ELA3B (CELA3B/1811), CF488A Conjugate

Produktmerkmale: This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CELA3B protein fragment (aa82-238) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: ELISA 2-4 μg/mL for coating (order Ab without BSA)

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Panc-28 or PANC1 cells. Pancreas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Andere Bezeichnung: CELA3B / ELA3B

Hintergrund: Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E

Molekulargewicht: 17 kDa

Gen-ID: 23436, 728752

UniProt: P08861