Elastase 3B Antikörper (AA 82-238) (CF®594)
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- Target Alle Elastase 3B (ELA3B) Antikörper anzeigen
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
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Bindungsspezifität
- AA 82-238
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Elastase 3B Antikörper ist konjugiert mit CF®594
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Applikation
- ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (IHC)
- Verwendungszweck
- Mouse Monoclonal anti-CELA3B / ELA3B (CELA3B/1257), CF594 Conjugate
- Produktmerkmale
- This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human CELA3B protein fragment (aa82-238) (exact sequence is proprietary)
- Klon
- CELA3B-1257
- Isotyp
- IgG1
- Top Product
- Discover our top product ELA3B Primärantikörper
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- Applikationshinweise
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Panc-28 or PANC1 cells. Pancreas
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
- Andere Bezeichnung
- CELA3B / ELA3B (ELA3B Produkte)
- Synonyme
- CBPP antikoerper, E1 antikoerper, EL-1 antikoerper, ELA3B antikoerper, 0910001F22Rik antikoerper, 2310074F01Rik antikoerper, AI504000 antikoerper, Ela3 antikoerper, Ela3b antikoerper, ela3b antikoerper, MGC82867 antikoerper, ELA1 antikoerper, Cela3b antikoerper, CELA3B antikoerper, chymotrypsin like elastase family member 3B antikoerper, chymotrypsin-like elastase family, member 3B antikoerper, elastase 3B, pancreatic antikoerper, proproteinase E antikoerper, chymotrypsin like elastase family member 3B L homeolog antikoerper, chymotrypsin-like elastase family member 3B antikoerper, CELA3B antikoerper, Cela3b antikoerper, ela3b antikoerper, LOC478196 antikoerper, cela3b.L antikoerper, LOC100717936 antikoerper, LOC100071692 antikoerper
- Hintergrund
- Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E
- Molekulargewicht
- 17 kDa
- Gen-ID
- 23436, 728752
- UniProt
- P08861
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