ESR2 Antikörper (C-Term) (Biotin)

Produktnummer ABIN6190591

Dieses Anti-ESR2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ESR2 in WB, IF, FACS und IHC (f). Geeignet für Human, Maus, Ratte, Schaf, Schwein, Pferd und Affe.

Kurzübersicht für ESR2 Antikörper (C-Term) (Biotin) (ABIN6190591)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human, Maus, Ratte, Schaf, Schwein, Pferd, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESR2 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: ERb455

Produktdetails anti-ESR2 Antikörper (Biotin)

Bindungsspezifität: C-Term

Verwendungszweck: Mouse Monoclonal anti-Estrogen Receptor beta 1 (ERb455), Biotin Conjugate

Produktmerkmale: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: C-terminus fragment of recombinant human estrogen receptor beta protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7 Cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: Estrogen Receptor beta 1

Hintergrund: Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor 2 (ER beta), estrogen receptor beta 4

Molekulargewicht: 53-59 kDa

Gen-ID: 2100, 660607

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling