pan Keratin Antikörper (CF®640R)

Produktnummer ABIN6190224

Der Maus Monoklonal Anti-pan Keratin-Antikörper wurde für IF, FACS, WB und IHC (f) validiert. Er ist geeignet, pan Keratin in Proben von Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen und Huhn zu detektieren.

Kurzübersicht für pan Keratin Antikörper (CF®640R) (ABIN6190224)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist konjugiert mit CF®640R

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: AE-1-AE-3

Produktdetails anti-pan Keratin Antikörper (CF®640R)

Verwendungszweck: Mouse Monoclonal anti-Cytokeratin, pan (AE-1 / AE-3), CF640R Conjugate

Produktmerkmale: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human epidermal keratin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Skin, Adeno- or Squamous carcinomas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Cytokeratin, pan

Hintergrund: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Molekulargewicht: 40-67 kDa

Gen-ID: 374454, 51350