pan Keratin Antikörper (CF®488A)

Produktnummer ABIN6190166

Dieses Maus Monoklonal-Antikörper erkennt spezifisch pan Keratin in IF, FACS, WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Rind (Kuh), Schwein, Frosch, Meerschweinchen, Ziege und Marmoset.

Kurzübersicht für pan Keratin Antikörper (CF®488A) (ABIN6190166)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein, Frosch, Meerschweinchen, Ziege, Marmoset

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist konjugiert mit CF®488A

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: C11

Produktdetails anti-pan Keratin Antikörper (CF®488A)

Verwendungszweck: Mouse Monoclonal anti-Cytokeratin, multi (C11), CF488A Conjugate

Produktmerkmale: This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Keratin-enriched preparation from cultured human A431

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 0.5-1.0 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A431 cells, Skin, Colon carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Cytokeratin, multi

Hintergrund: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Molekulargewicht: Multiple

Gen-ID: 3851, 3852, 3853