ALDH1A1 Antikörper (AA 315-434) (CF®594)

Produktnummer ABIN6190130

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ALDH1A1 in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ALDH1A1 Antikörper (AA 315-434) (CF®594) (ABIN6190130)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ALDH1A1 Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: ALDH1A1-1381

Produktdetails anti-ALDH1A1 Antikörper (CF®594)

Bindungsspezifität: AA 315-434

Verwendungszweck: Mouse Monoclonal anti-ALDH1A1 (ALDH1A1/1381), CF594 Conjugate

Produktmerkmale: ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant fragment of human ALDH1A1 (aa 315-434) (exact sequence is proprietary)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HepG2 or A549 cells. Colon or Testicular Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Andere Bezeichnung: ALDH1A1

Molekulargewicht: 56 kDa

Gen-ID: 216, 76392

UniProt: P00352

Pathways: Dopaminergic Neurogenesis