TYR Antikörper (CF®647)

Produktnummer ABIN6189734

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TYR in IF, ELISA, FACS, IHC (f) und Coat. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TYR Antikörper (CF®647) (ABIN6189734)

Target: TYR (Tyrosinase (TYR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYR Antikörper ist konjugiert mit CF®647

Applikation: Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Klon: OCA1-812

Produktdetails anti-TYR Antikörper (CF®647)

Verwendungszweck: Mouse Monoclonal anti-Tyrosinase (OCA1/812), CF647 Conjugate

Produktmerkmale: This antibody recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TYR protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, or 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• ELISA For coating, order Ab without BSA
• Optimal dilution for a specific application should be determined by user

Kommentare:

SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu TYR

Target: TYR (Tyrosinase (TYR))

Andere Bezeichnung: Tyrosinase

Hintergrund: ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR

Molekulargewicht: 70-80 kDa

Gen-ID: 7299, 503555

UniProt: P14679