ODC1 Antikörper (CF®647)

Produktnummer ABIN6188898

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ODC1 in WB, IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für ODC1 Antikörper (CF®647) (ABIN6188898)

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ODC1 Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: ODC1-486

Produktdetails anti-ODC1 Antikörper (CF®647)

Verwendungszweck: Mouse Monoclonal anti-ODC-1 (Ornithine Decarboxylase 1) (ODC1/486), CF647 Conjugate

Produktmerkmale: This antibody recognizes a 53 kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ODC-1 protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

PC3 cells. Placenta, Prostate or Testicular carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu ODC1

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Andere Bezeichnung: ODC-1 (Ornithine Decarboxylase 1)

Hintergrund: Ornithine decarboxylase structural 1, RNODC

Molekulargewicht: 53 kDa

Gen-ID: 4953, 467701

UniProt: P11926