MAML3 Antikörper (CF®594)

Produktnummer ABIN6188154

Produktdetails anti-MAML3 Antikörper (CF®594)

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAML3 Antikörper ist konjugiert mit CF®594

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt)

Verwendungszweck: Mouse Monoclonal anti-MAML3 (MAML3/1303), CF594 Conjugate

Produktmerkmale: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J?, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length MAML3 protein

Klon: MAML3-1303

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Andere Bezeichnung: MAML3 (MAML3 Produkte)

Synonyme: AV234550 antikoerper, BC049812 antikoerper, Mam-2 antikoerper, mKIAA1816 antikoerper, Glrp1 antikoerper, CAGH3 antikoerper, ERDA3 antikoerper, GDN antikoerper, MAM-2 antikoerper, MAM2 antikoerper, TNRC3 antikoerper, mastermind like 3 (Drosophila) antikoerper, mastermind-like transcriptional coactivator 3 antikoerper, mastermind like transcriptional coactivator 3 antikoerper, Maml3 antikoerper, MAML3 antikoerper

Hintergrund: CAGH3, ERDA3, GDN, MAML3, Mastermind-like protein 3, TNRC3

Molekulargewicht: 150-170 kDa

Gen-ID: 55534