CD63 Antikörper
-
- Target Alle CD63 Antikörper anzeigen
- CD63
-
Reaktivität
- Human, Maus
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser CD63 Antikörper ist unkonjugiert
-
Applikation
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-CD63 (MX-49.129.5), Purified, BSA-free
- Produktmerkmale
- This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Aufreinigung
- Purified
- Immunogen
- Full length CD63 of human origin
- Klon
- MX-49-129-5
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product CD63 Primärantikörper
-
-
- Applikationshinweise
-
Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
-
SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Konservierungsmittel
- Azide free
-
- Target
- CD63
- Andere Bezeichnung
- CD63 (CD63 Produkte)
- Synonyme
- C75951 antikoerper, ME491 antikoerper, Tspan30 antikoerper, LAMP-3 antikoerper, MLA1 antikoerper, OMA81H antikoerper, TSPAN30 antikoerper, TSP29FA antikoerper, CD63 antigen antikoerper, CD63 molecule antikoerper, Cd63 molecule antikoerper, Cd63 antikoerper, CD63 antikoerper
- Molekulargewicht
- 26 kDa (core protein), 30-60 kDa (glycosylated)
- Gen-ID
- 967, 445570
- UniProt
- P08962
-