CD81 Antikörper (CF®405S)

Produktnummer ABIN6187142

Dieses Anti-CD81-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD81 in FACS, WB, IHC, IF und Func. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CD81 Antikörper (CF®405S) (ABIN6187142)

Target: CD81

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD81 Antikörper ist konjugiert mit CF®405S

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func)

Klon: 1-3-3-22

Produktdetails anti-CD81 Antikörper (CF®405S)

Verwendungszweck: Mouse Monoclonal anti-CD81 / TAPA-1 (1.3.3.22), CF405S Conjugate

Produktmerkmale: This antibody This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: B-Cell line derived from a Burkitt lymphoma

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: For functional studies, order Ab without azide

• Immunohistology (formalin) 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, and human lymphocytes. Tonsil or Liver.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD81

Target: CD81

Andere Bezeichnung: CD81 / TAPA-1

Hintergrund: CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28

Molekulargewicht: 26 kDa

Gen-ID: 975, 54457

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C