Rekombinanter CD34 Antikörper (Biotin)

Produktnummer ABIN6185955

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch CD34 in FACS, WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für Rekombinanter CD34 Antikörper (Biotin) (ABIN6185955)

Target: CD34

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: HPCA1-1806R

Produktdetails anti-CD34 Antikörper (Biotin)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-CD34 (HPCA1/1806R), Biotin Conjugate

Produktmerkmale: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi s sarcoma, but shows low specificity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human HPCA1 protein

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Immunohistology (formalin) 2-4 μg/mL
• Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

KG-1 cells, Tonsil, or Angiosarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

Molekulargewicht: 90-110 kDa

Gen-ID: 947, 374990

UniProt: P28906