TDP2 Antikörper (CF®405S)
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- Target Alle TDP2 Antikörper anzeigen
- TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser TDP2 Antikörper ist konjugiert mit CF®405S
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Applikation
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- Mouse Monoclonal anti-TDP2 / EAPII (TDP2/1258), CF405S Conjugate
- Produktmerkmale
- This MAb recognizes a protein of 41 kDa, which is identified as TDP2. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human full-length TDP2 protein
- Klon
- TDP2-1258
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product TDP2 Primärantikörper
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- Applikationshinweise
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Jurkat cells. Lung, Duodenum or Small Intestinal Carcinoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))
- Andere Bezeichnung
- TDP2 / EAPII (TDP2 Produkte)
- Synonyme
- AD022 antikoerper, EAP2 antikoerper, EAPII antikoerper, TTRAP antikoerper, dJ30M3.3 antikoerper, hTDP2 antikoerper, ad022 antikoerper, eap2 antikoerper, ttrap antikoerper, D13Ertd656e antikoerper, Ttrap antikoerper, tdp2 antikoerper, ttrapl antikoerper, tyrosyl-DNA phosphodiesterase 2 antikoerper, tyrosyl-DNA phosphodiesterase 2 S homeolog antikoerper, tyrosyl-DNA phosphodiesterase 2b antikoerper, TDP2 antikoerper, tdp2.S antikoerper, Tdp2 antikoerper, tdp2b antikoerper
- Molekulargewicht
- ~41 kDa
- Gen-ID
- 51567
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