MUC1 Antikörper (Biotin)

Produktnummer ABIN6184169

Dieses Anti-MUC1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MUC1 in FACS, IF und IHC (f). Geeignet für Human.

Kurzübersicht für MUC1 Antikörper (Biotin) (ABIN6184169)

Target: MUC1 (Mucin 1 (MUC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: E29

Produktdetails anti-MUC1 Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-Mucin 1 / EMA / Episialin / CD227 (E29), Biotin Conjugate

Produktmerkmale: In Western blotting, this antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Delipidated extract of human milk fat globule membranes

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: Mucin 1 / EMA / Episialin / CD227

Molekulargewicht: 265-400 kDa

Gen-ID: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling