MUC1 Antikörper (Biotin)

Produktnummer ABIN6184131

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MUC1 in ELISA, FACS, IF, IHC (f) und Coat. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für MUC1 Antikörper (Biotin) (ABIN6184131)

Target: MUC1 (Mucin 1 (MUC1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Klon: 139H2

Produktdetails anti-MUC1 Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-Mucin 1 / EMA / Episialin / CD227 (139H2), Biotin Conjugate

Produktmerkmale: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human milk-fat globule membranes (HMFGM)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 1-2 μg/mL
• ELISA For coating use Ab at 2-5 μg/mL, order Ab without BSA
• Flow Cytometry 0.25-0.5 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: Mucin 1 / EMA / Episialin / CD227

Molekulargewicht: 265-400 kDa

Gen-ID: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling