CA9 Antikörper (Biotin)

Produktnummer ABIN6182193

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CA9 in WB, IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human und Pferd.

Kurzübersicht für CA9 Antikörper (Biotin) (ABIN6182193)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reaktivität: Human, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CA9 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CA9-781

Produktdetails anti-CA9 Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-Carbonic Anhydrase IX (CA9/781), Biotin Conjugate

Produktmerkmale: This antibody recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CA9 protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Normal kidney or renal cell carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Andere Bezeichnung: Carbonic Anhydrase IX

Molekulargewicht: 55 kDa

Gen-ID: 768, 63287

UniProt: Q16790