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Myosin (Smooth Muscle Heavy Chain) Antikörper (CF®647)

Reaktivität: Human, Ratte FACS, IF, IHC (f) Wirt: Maus Monoclonal MYH11-923 SMMS-1 CF®647
Produktnummer ABIN6180082
  • Target Alle Myosin (Smooth Muscle Heavy Chain) Produkte
    Myosin (Smooth Muscle Heavy Chain)
    Reaktivität
    • 18
    • 17
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    Human, Ratte
    Wirt
    • 18
    Maus
    Klonalität
    • 18
    Monoklonal
    Konjugat
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser Myosin (Smooth Muscle Heavy Chain) Antikörper ist konjugiert mit CF®647
    Applikation
    • 17
    • 17
    • 17
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Verwendungszweck
    Mouse Monoclonal anti-Myosin, Smooth Muscle Heavy chain (MYH11/923 + SMMS-1), CF647 Conjugate
    Produktmerkmale
    Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human MYH11 protein (MYH11/923), Human uterus extract (SMMS-1)
    Klon
    MYH11-923 SMMS-1
    Isotyp
    IgG
  • Applikationshinweise
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Predicted to show broad species reactivity
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    Uterus or normal breast

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Protect from light
  • Target
    Myosin (Smooth Muscle Heavy Chain)
    Andere Bezeichnung
    Myosin, Smooth Muscle Heavy chain (Myosin (Smooth Muscle Heavy Chain) Produkte)
    Hintergrund
    AAT4, Myosin heavy chain 11 (MYH11), Myosin heavy chain 11 smooth muscle, Smooth muscle myosin heavy chain 11, Myosin-11, SM1, SM2, SMHC, SMMHC
    Molekulargewicht
    205 kDa (MHC-1) and 200 kDa (MHC-2)
    Gen-ID
    4629, 460109
    UniProt
    P35749
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