KRT8 Antikörper (CF®594)

Produktnummer ABIN6178958

Dieses Anti-KRT8-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von KRT8 in IF, FACS und IHC (f). Geeignet für Human und Ratte.

Kurzübersicht für KRT8 Antikörper (CF®594) (ABIN6178958)

Target: KRT8 (Keratin 8 (KRT8))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT8 Antikörper ist konjugiert mit CF®594

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: H1

Produktdetails anti-KRT8 Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Cytokeratin 8 (H1), CF594 Conjugate

Produktmerkmale: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cytoskeleton preparation containing cytokeratin 8

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu KRT8

Target: KRT8 (Keratin 8 (KRT8))

Andere Bezeichnung: Cytokeratin 8

Hintergrund: CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, KRT8, Type-II Keratin Kb8

Molekulargewicht: 52.5 kDa

Gen-ID: 3856

UniProt: P05787