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Rekombinanter Keratin Basic Antikörper (CF®405S)

Reaktivität: Human WB, IF, IHC, FACS Wirt: Kaninchen Monoclonal KRTH-1576R CF®405S Recombinant Antibody
Produktnummer ABIN6178782
  • Target Alle Keratin Basic Antikörper anzeigen
    Keratin Basic
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 21
    • 13
    • 10
    • 8
    • 8
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 14
    • 8
    Kaninchen
    Klonalität
    • 22
    Monoklonal
    Konjugat
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    CF®405S
    Applikation
    • 19
    • 17
    • 15
    • 13
    • 10
    • 5
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Verwendungszweck
    Recombinant Rabbit Monoclonal anti-Cytokeratin, Basic (KRTH/1576R), CF405S Conjugate
    Produktmerkmale
    This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant full-length human KRT76 protein
    Klon
    KRTH-1576R
    Isotyp
    IgG kappa
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  • Applikationshinweise
    Immunohistology (formalin) 0.25-0.5 μg/mL
    • Immunofluorescence 1-2 μg/mL Western blotting 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    Epithelial cells, Skin or Adenocarcinomas

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Protect from light
  • Target
    Keratin Basic
    Andere Bezeichnung
    Cytokeratin, Basic (Keratin Basic Produkte)
    Hintergrund
    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
    Molekulargewicht
    52-67 kDa
    Gen-ID
    51350, 654392
    UniProt
    Q01546
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