Rekombinanter CNN1 Antikörper

Produktnummer ABIN6178363

Der Kaninchen Monoklonal Anti-CNN1-Antikörper wurde für FACS, IF und IHC validiert. Er ist geeignet, CNN1 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für Rekombinanter CNN1 Antikörper (ABIN6178363)

Target: CNN1 (Calponin 1 (CNN1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CNN1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: CNN1-1408R

Produktdetails anti-CNN1 Antikörper

Verwendungszweck: Recombinant Rabbit Monoclonal anti-Calponin-1 (CNN1/1408R), Purified, BSA-free

Produktmerkmale: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Recombinant human full-length CNN1 protein

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA  pH 7.5-8.5 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Myoepithelial cells in breast ducts

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu CNN1

Target: CNN1 (Calponin 1 (CNN1))

Andere Bezeichnung: Calponin-1

Hintergrund: Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC

Molekulargewicht: 34 kDa

Gen-ID: 1264, 465929

UniProt: P51911